multi-array chemokine cytokine assays  (Meso Scale Diagnostics LLC)

Journal: International Journal of Molecular Sciences

Article Title: The Iron Chelator Desferrioxamine Increases the Efficacy of Bedaquiline in Primary Human Macrophages Infected with BCG

doi: 10.3390/ijms22062938

Figure Lengend Snippet: Determining the effect of combined DFX-bedaquiline treatment on cytokine secretions in hMDMs infected with BCG. hMDMs, obtained from healthy blood donors, were infected with BCG for three hours and were subsequently treated with DFX (100 µM) in combination with bedaquiline (0.5 µg/mL). Twenty-four hours post BCG infection, cell supernatants were collected and levels of ( A ) IFNγ, ( B ) IL-6, ( C ) IL-1β, ( D ) IL-8, ( E ) IL-13, ( F ) IL-2, ( G ) IL-4, ( H ) IL-10, ( I ) IL12p70 and ( J ) TNF-α assayed using Meso Scale Discovery Multi-Array technology ( n = 5). ( K ) DFX increases the bactericidal/bacteriostatic properties of bedaquiline in primary hMDMs infected with BCG. Bars denote mean ± SEM. * p < 0.05 and ** p < 0.01 (Two-way repeated measures ANOVA tests with Šídák’s multiple comparisons tests).

Article Snippet: Meso Scale Discovery Multi-Array technology chemokine and cytokine assays were statistically analysed using two-way repeated measures ANOVA tests with Šídák’s multiple comparisons tests.

Techniques: Infection

Journal: International Journal of Molecular Sciences

Article Title: The Iron Chelator Desferrioxamine Increases the Efficacy of Bedaquiline in Primary Human Macrophages Infected with BCG

doi: 10.3390/ijms22062938

Figure Lengend Snippet: Assessing if dual DFX-bedaquiline treatment affects chemokine secretions in BCG-infected hMDMs. hMDMs, obtained from healthy blood donors, were infected with BCG for three hours and were subsequently treated with DFX (100 µM) in combination with bedaquiline (0.5 µg/mL). Twenty-four hours post BCG infection, cell supernatants were collected and levels of ( A ) eotaxin, ( B ) eotaxin-3, ( C ) TARC, ( D ) IP-10, ( E ) MCP-1, ( F ) MCP-4, ( G ) MDC, ( H ) MIP-1α and ( I ) MIP-1β were quantified using Meso Scale Discovery Multi-Array technology ( n = 5). Bars denote mean ± SEM. p > 0.05 (Two-way repeated measures ANOVA tests with Šídák’s multiple comparisons tests).

Article Snippet: Meso Scale Discovery Multi-Array technology chemokine and cytokine assays were statistically analysed using two-way repeated measures ANOVA tests with Šídák’s multiple comparisons tests.

Techniques: Infection

Journal: Laser Therapy

Article Title: Modulation of inflammatory response of wounds by antimicrobial photodynamic therapy

doi: 10.5978/islsm.15-OR-13

Figure Lengend Snippet: Effect of PDT on expression of proinflammatory cytokines measured in wound tissue homogenates day 5 p.w.by ELISA. (U): Uninfected, (I): Untreated infected and (P): Infected wounds treated with pl-cp6 and then exposed to red light (660 ± 25 nm) ...

Article Snippet: Cytokine ELISA The levels of inflammatory cytokines IL-1α, IL-1β, and IL-2 in wound tissues of different groups excised on day 5 p.w. were measured using a mouse inflammatory cytokines and chemokines multi analyte™ ELISA array kit (SA Biosciences Corp, USA).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Infection